Jan Havliš, Ph.D.
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National Centre for Biomolecular Research Laboratory of Functional Genomics and Proteomics
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Central European Institute of Technology Mendel Centre for Plant Genomics and Proteomics Proteomics Core Facility
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****science never sleeps****
****mass spec never lies****
telephone: +420-54949-4148
facsimile: +420-54949-2654
e-mail: jdqh(at)chemi(dot)muni(dot)cz, jdqh(at)sci(dot)muni(dot)cz
university Information System (IS) web-page
personal web-page
quick-navigation: [LECTURES ] [LAB-COURSES ] [SEMINARS ] [STUDENTS ] [PROJECTS ]
C7030 Separacni metody (in Czech; Separation methods) (IS web-page):
autumn semester; 2 hours per week / 2+2 credits
topics: liquid-liquid extraction, liquid-solid extraction, sample preparation, liquid chromatography, evaluation of the separation, chiral separations, separation of macromolecules (size exclusion, flow-field fractionation), gas chromatography, electromigration methods, separation method validation.
lecture guide (in Czech) : on IS as a study material when actual
C7023 Separation methods A (in English) (IS web-page):
autumn semester; 2 hours per week / 2+2 credits
topics: liquid-liquid extraction, liquid-solid extraction, sample preparation, liquid chromatography, evaluation of the separation, chiral separations, separation method validation.
lecture guide (in English) : on IS as a study material when actual
C8024 Separation methods B (in English) (IS web-page):
spring semester; 2 hours per week / 2+2 credits
topics: separation of macromolecules (size exclusion, flow-field fractionation), gas chromatography, electromigration methods.
lecture guide (in English) : on IS as a study material when actual
Bi7942 Bioanalytika I - Biomakromolekuly (in Czech) (IS web-page):
Bi7940 Bioanalytics I - Biomacromolecules (in English) (IS web-page):
autumn semester; 2 hours per week / 2+2 credits
topics: biomacromolecular analytics: principles and applications; affinity methods, immunoseparations, protein complex analysis, DNA analysis, lipid analysis.
lecture guide (in Czech or in English) : on IS as a study material when actual
Bi7072 Bioanalytika II - Analytické metody v klinicke praxi (in Czech) (IS web-page):
Bi7071 Bioanalytics II - Analytical methods in clinical praxis (in English) (IS web-page):
spring semester; 2 hours per week / 2+2 credits
topics: clinical chemistry and analytical methods (world trends, number of analyses, instruments). the specifity of biological samples (matrix effects, interferences and stability of the main analytes). drawing, transport and storage conditions of biological samples. the compatibility of reaction media with biological sample and analyte to be determined. the examples for developing and performing analytical methods for the determination of ions, enzymes, substrates, proteins and lipids. automation in clinical chemistry, standards, control materials, internal and external quality control.
lecture guide (in Czech or in English) : on IS as a study material when actual
both semesters; 2 hours per week / 2+2 credits
topics: science as a method (what all it could be and what not, its history, philosophy, epistemology, language, methods and tools of discovering, procedure of science. science as a part of social cognisance (its role in society, applications, technology, popularisation, ethics and character of scientific work. science as a system (scientific information, publication and communication, scientometry, importance of scientific lingua franca, scientific community, fundings.
lecture guide (in Czech) : on IS as a study material when actual
C6242 Analyza organickych latek II (in Czech; Analysis of organic compunds II ) (IS web-page):
principal teacher
: Dr. Farkova
spring semester; 2 hours per week (4 weeks) / 2+2 credits
topics: colourness, basics of theory and examples of applications: basical indicator reactions (azocoupling, oxidative coupling. immunoanalytical chemistry: classification of the methods; competitive and noncompetitive modes, homogenic and heterogenic methods. analytical importance of nucleic acids, structure and properties, target NA sequence. PCR and its utilization for patogenic, forensic, prenatal diagnostics. analysis of proteins: spectrophotometrical methods, basics of mass spectrometric approaches and enzymatic analysis.
lecture guide (in Czech) : on IS as a study material when actual
LABORATORY COURSES
N / A
N / A
PhD STUDENTS
David Potesil (2006/2010) - Study of molecular markers of B-cellular chronic lymphocytic leukaemia
Hana Ovadova (2007/2011) - New approaches in metalloprotein analysis
Radek Pilny (2007/2011) - Optimisation of SELDI/MALDI TOF MS based approaches in detection of putative soluble oncomarkers
MASTER DEGREE STUDENTS
Marcela Zupkova (2009/2011) - Staphylococcal enterotoxin determination by means of mass spectrometry
BACHELOR DEGREE STUDENTS
N / A
Major Research Projects
Proteins in metabolism and within interaction of organism with environment, Masaryk University, NCBR, Brno, since 2005, Prof Koca, Prof Kucera
Molecular basis of cell and tissue regulations, Masaryk University, Institute of Experimental Biology, Brno, since 2004, Prof Fajkus
Research Projects
Modern methods of detection and determination of microbial toxins, Grant Agency of Czech Republic, Czech Republic, since 2009, Prof Vytrasova
Co-laborations
Protein engineering of proteolytic enzymes used in MS-driven proteomics, Masaryk University, Institute of Experimental Biology, Brno and Palacky University, Department of Biochemistry, Olomouc, since 2001, Prof Marek Sebela